Coding

Part:BBa_K1861201:Design

Designed by: Vladyslav Vyshnevskyi   Group: iGEM15_FAU_Erlangen   (2015-09-18)

TALE constant domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 991
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 787
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 442
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a component of BBa_K1861020.
In order to insert the variable TALE domain into the constant domain, we had to introduce two restriction sites for Esp3I as well as a spacer between them.

Source

The sequence was obtained using the TAL Plasmids Sequence Assembly Tool.

References

http://bao.rice.edu/Research/BioinformaticTools/assembleTALSequences.html, visited on 2015-09-20 http://web.archive.org/web/20150920175258/http://bao.rice.edu/Research/BioinformaticTools/assembleTALSequences.html